Control Peptide for C1075-70 (affintiy purified) and C1075-71B (antiserum).
Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana. (-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of cannabis. Cannabis affect cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics. To date, two sub-types of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified. CB1 (rat/mouse 473 aa, human 472 aa, ~95% sequence homology) has 7 TM domains with an extracellular N-terminus and cytoplasmic C-terminus. An alternative spliced N-terminal variant, CB1A, has also been reported. The CB1 receptor has been shown to inhibit adenylate cyclase activity in a pertussis-toxin sensitive manner and to be more responsive to psychoactive cannabinoids than to non-psychoactive cannabinoids. CB1 also couples to a pertussis-toxin sensitive G-protein to regulate Ca2+ currents, to activate inward rectifying K+ channels, and to activate MAP Kinases. CB1 receptor is distributed throughout the brain, with higher representation in the hippocampus, a brain region essential for storage of newly acquired information and changes of mood and behavioral state. In the cerebellum, CB1 is most dense in the molecular layer, consistent with the effects of cannabinoids on the coordination of motor function and motor learning. Outside the CNS, CB1 expression is found in testes, endothelial cells, and ileum longitudinal smooth muscle.
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution:ELISA: 50-100ng control peptide per well
Antibody Blocking: 5-10ug per 1ug C1075-70 (affintiy purified) or 1ul C1075-71B (antiserum)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Grade：Highly Purified
Purity：Highly Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：A 14 amino acid peptide sequence near the extracellular, N-terminus of human CB1. The immunogen shows no significant sequence homology with CB2 or other receptors. Species sequence homology: Rat, mouse, feline, monkey, zebra finch, newt (salamander): 100%; pufferfish: 85%.
