85-1384-71　Histamine Receptor 3, NT, Control Peptide (HR3) 100ug　H5090-20A

特徴

• Control peptide for H5090-20 (affinity purified antibody) and H5090-20B (antiserum).
• Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitter. It plays an important role in a multitude of physiological processes in central and peripheral tissues. Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g. sleep/wakefulness, hormonal secretion, cardiovascular control, thermoregulation, food intake, and memory formation). In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells. Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system. Histamine effects are mediated by four pharmacologically distinct receptors: the H1R, H2R , H3R and H4R receptors. These receptors are all members of the G-protein coupled receptor (GPCR) family. Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length. Human Histamine 3 receptor, H3R (GPCR97), has recently been identified as a presynaptic autoreceptor on histamine neurons in the brain controlling the stimulated release of histamine. The H3 receptor has been shown to be a presynaptic heteroreceptor in nonhistamine- containing neurons in both the central and peripheral nervous systems. H3R (rat/human 445aa, ~93% homology) is primarily expressed in the brain. Several studies using H3 selective agonists revealed that H3R couples to pertussis toxin-sensitive Gi/o protein.
• Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to its low molecular weight. Other applications not tested.
• Recommended Dilution:Antibody Blocking: 5-10ug per 1 ug H5090-20 (affinity purified antibody) or 1ul H5090-20B (antiserum)
• ELISA: 50-100ng control peptide per well. Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.