Control Peptide for F0019-68B (antiserum) and F0019-68 (affinity purified).
A 17 amino acid peptide sequence near the N-terminus of human FAAH. Peptide is 100% conserved in rat, 94% in mouse and porcine and 58% in chicken. No significant homology exists with other hydrolases.
Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana. (-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of cannabis. Cannabis affect cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics. To date, two sub-types of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified. The first brain-derived endogenous cannabinoid, an unsaturated fatty-acid ethanolamide, arachidonylethanolamide (AEA, also called anandamide) was found in brain. AEA has higher affinity for the CB1 than for the CB2. Neurons and astrocytes have been found to re-uptake and hydrolyse anandamide rapidly, resulting in the formation of arachidonic acid and ethanolamine. The uptake mechanism has been shown to be mediated by a saturable, selective, temperature-dependent and Na+-independent transporter. Anandamide hydrolysis is catalyzed by a membrane-bound amidohydrolase (called anandamide amidohydrolase or fatty acid amide hydrolase, FAAH). FAAH (rat/mouse/human 579 aa; chromosome 1p34-p35; mol wt ~67 kD) sequence analyses suggest a single predicted transmembrane domain at the extreme N-terminus of the enzyme. Distribution of FAAH parallels CB1 in rat brain suggesting that FAAH participates in cannabinoid signalling mechanisms. The sn-2-Arachidonylglycerol (2-AG), initially isolated from intestine, appears to be the second endogenous CB ligand (CB1 Ki 472 nM; CB2 Ki 1400 nM). 2-AG concentration in the brain is 170 times greater than anandamide. FAAH hydrolyzes 2-AG at a rate four times faster than that for anandamide hydrolysis.
Application(s): Suitable for use in ELISA, Antibody Blocking.
Recommended Dilution:ELISA: 50-100ng of control peptide/well
