Transcription factor CREB binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity (1,2). Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1 (3–5). CREB appears to play an important role in learning and memory in both flies and mice.
Produced by immunizing rabbits with a synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser133 of human CREB.
Corresponding antibody, C7915-10, detects endogenous levels of CREB only when phosphorylated at serine 133. Also detects the phosphorylated form of CREB-related protein ATF-1. Species Crossreactivity: Mouse and rat.
Applications:Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
