Recombinant Human Vascular Endothelial Cell Growth Factor (VEGF) is a dimer (composed of two 22kD chains, each chain containing 165 amino acid residues). Produced from recombinant human DNA expressed in baculovirus infected Sf21cells. Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 38.2kD protein consisting of two 165 amino acid polypeptide chains.
Cell Proliferation Assay: VEGF activity was determined as the ability to stimulate proliferation of human umbilical vein endothelial cells (HUVE cells) during a five day growth assay. In the assay described below, this lot at 2ng/ml stimulated cell proliferation 1.4fold over the untreated control. Results may vary depending on the cell line used. VEGF will also stimulate endothelial cells from other species.
Dilution: VEGF is acid stable. Dilute in either 0.1% TFA or 4mM HCl, containing 25ug BSA per ug VEGF to maintain stability. Reducing agents (such as dithiothreitol or 2-mercaptoethanol) inhibit VEGF activity.
Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
仕様
Size：10ug
Source Antigen：Recombinant, Sf21 cells
Grade：Highly Purified
Purity：Ion exchange chromatography followed by 0.2um filter sterilization.
Form：Supplied as a frozen liquid in 25mM HEPES, pH 7.0, 150mM sodium chloride. Carrier free.
