Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine TH2 clones that suppressed the production of cytokines by TH1 clones responding to stimulation by antigen in the presence of monocyte/macrophage antigen-presenting cells. The human homolog of murine IL-10 was subsequently cloned by cross-hybridization. Human and murine IL-10 are 81% and 73% identical at the nucleotide and amino acid levels, respectively. Both mouse and human IL-10 are also highly homologous to a previously uncharacterized open reading frame in the Epstein-Barr virus (EBV) genome, BCRF1. The BCRF1 gene product is now designated viral IL-10. In mouse, IL-10 is produced by TH2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1+ (CD5+) and normal B cells. Cell lines found to have the capacity to produce IL-10 in humans include: TH0, TH1, and TH2-like CD4+ T cell clones; B cell lines derived from patients with AIDS and Burkitt‘s lymphoma; activated monocytes and peripheral blood T cells, including TH2-like CD8; CD4+CD45RA naïve and CD4+CD45RA memory T cells, plus bronchogenic carcinoma cells.
Specificity:Recognizes both natural and recombinant human IL-10 and exhibits no significant crossreactivity with human IL-1a, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNF-a, TNF-b, IFN-g. There is ~2% reaction with recombinant mouse IL-10.
Kit Components:I8432-05A: Microtiter Plate 1x96 wellsI8432-05B: Pab (Biotin) 1x7mlI8432-05C: Avidin (HRP) 1x14ml*I8432-05D: Standard 2x1vialI8432-05E: Calibrator Diluent I 1x22mlI8432-05F: Calibrator Diluent II (5X) 1x22mlI8432-05G: Wash Buffer (20X) 1x60mlI8432-05H: Substrate A 1x10mlI8432-05J: Substrate B 1x10mlI8432-05K: Stop Solution 1x14ml
Storage and Stability:Store *I8432-05D powder at 4°C, liquid at -20°C. Stable for 30 days. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
