Peptide (CQYQAPDMDTLQIHVME) corresponding to amino acids 400 to 416 of human IKKg (1,2), which are identical to those of mouse homologue (2).
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The IkB kinase (IKKa and IKKb) phosphorylates IkB and mediates NF-kB activation. A novel molecule in the IKK complex was recently identified and termed IKKg/NEMO/FIP3/IKKAP1 (1-5). IKKg interacts with IKKb and is required for the activation of IKK complex and NF-kB by LPS, PMA, TNF, and IL-1 stimulation (1-4). FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kB activation(3).
Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.
Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
