36kD protein ubiquitously expressed in many cell types. The eIF-2 protein, which is composed of three subunits (a, b and g), is one of the key molecules in the initiation of translation. The phosphorylation of eIF-2a is an important regulatory process in protein synthesis. In mammalian cells, eIF-2a is phosphorylated at serine 51 by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF-2a blocks the GDP-GTP exchange activity of eIF-2b, resulting in the suppression of protein synthesis.
P9108-48A1:Protein Synthesis Initiation Factor 2a (pS51), Positive Control Peptide
Source:Synthetic peptide
MW:~1591D
Purity:~90% by HPLC.
Form:Supplied as a white to off-white lyophilized powder.
AA Sequence:H-Ile-Leu-Leu-Ser-Glu-Leu-{pSer}-Arg-Arg-Arg-Ile-Arg-OH
P9108-48A2:Protein Synthesis Initiation Factor 2a (pS51), Negative Control Peptide
Source:Synthetic peptide
MW:~1512D
Purity:~90% by HPLC.
Form:Supplied as a white to off-white lyophilized powder.
AA Sequence:H-Ile-Leu-Leu-Ser-Glu-Leu-Ser-Arg-Arg-Arg-Ile-Arg-OH
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
