Vitronectin (S-protein) is a multi-functional plasma and extracellular matrix protein with defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to other macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration.
Applications:Suitable for use in ELISA, Immunization, Cell Attachment Assays and Electrophoresis. Other applications not tested..
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Activity:Active in cell attachment assays at 1ug/ml. Binds to heparin. Active in cell attachment of MG63 cells.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Human plasma, tested negative for antibodies to HBsAg and HIV.
Grade：Purified
Purity：≥90% (SDS-PAGE). Two bands in SDS-PAGE at 65kD and 75kD.
Form：Supplied as a liquid in 50mM sodium phosphate, 100mM sodium chloride, pH 7.4. No preservatives added.
