H-MMP-7 is a member of the Matrix metalloproteinase family of endopeptidases, containing the canonical HExxHxxGxxH motif as a zinc binding active site. MMP-7 and MMP-26 (Matrilysin-2) are the only MMPs discovered that completely lack a hemopexin “regulatory domain” or hinge region. Like the other MMPs, MMP-7 is secreted as a zymogen, then activated. The 28kD form is reduced to 18kD by enzymatic cleavage after the conserved “cysteine switch.” MMP-7 is not constitutively produced. It is produced on demand in specific tissues. MMP-7 substrate specificity is broad, most closely resembling the activity of MMP-3 (Stromelysin-1). When MMP-7 production is stimulated, native MMP inhibitors (TIMPs) usually follow to quench them.
Applications:Suitable for use in ELISA, Western Blot (control or standard) and SDS-PAGE Standards. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Molecular Weight:19,130 calculated from amino acid sequence and 20,000 from SDS-PAGE, followed by staining with CBB R-250.
Specific Activity:~5000 U/mg protein
Unit Definition:One unit of enzyme activity is defined as the quantity required to digest 1ug of Azocoll/min. at pH 7.5 and 37°C.
Storage and Stability:MMP-7, when stripped of TIMPs, is unstable, and must stored carefully. To avoid repeated freeze thaw cycles, aliquot and store the enzyme at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Form：Supplied as a liquid in 10mM HEPES, pH 7.4, 5mM calcium chloride, 0.15M sodium chloride.
