Laminin is a major component of basement membranes and stimulates neurite outgrowth, promotes cell attachment, chemotaxis and cell differentation. Promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue.
Applications:Suitable for use in ELISA, production of antiserum, cell adhesion assays, and neurite-stimulation assays. Active in cell attachment.
Recommended Dilution:1-10ug/cm2 of growth surface depending on cell type
The optimal laminin concentration may vary with the cell type and has to be determined experimentally.
MW: 900kD (unreduced)
Quality Control:Tested for ability to initiate differentiation (neurite outgrowth) of NG-108 human neuroblastoma cells.
Specifications: 3 chains (reduced) at 220, 230, 400kD on SDS-PAGE.
Storage and Stability:Store at-20°C. Thaw carefully at RT and do not refreeze! Store thawed material at 2–8°C for up to 3 months.
仕様
Size：1mg
Source Antigen：Engelbreth-Holm-Swarm mouse tumor.
Grade：Purified
Purity：Purified as laminin-nidogen complex from EHS sarcoma according to the method of Timpl, et al. (1,2). ≥ 90% by SDS-PAGE.
Form：Supplied as a liquid in sterile 0.05M Tris HCl, pH 7.4, 0.15M sodium chloride, 2mM EDTA. No preservative added.
