Integrin alpha V beta 3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alpha V beta 3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described (1). Product was tested and found negative for HIV and Hepatitis.
Functional Activity:Confirmed by vWf (von Willibrand factor) binding assay. It is expected that this preparation of alphaV beta3 integrin will also interact with other alphaV beta3 binding proteins in an RGD-dependent fashion.
Applications:Suitable for use in Electrophoresis and as Western Blot control. Also useful for in vitro binding experiments with metalloproteinase MMP-2 as well as functional evaluations. Other applications not tested.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Source Antigen：Human placenta
Grade：Highly Purified
Purity：≥95% by SDS-PAGE (silver stain). Purified by affinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.4, 100mM Octyl-b-D-glucopyranoside, 0.09% sodium azide.
