Mouse IgG1 is a monoclonal antibody intended for use as a negative control. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.
Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry (Frozen and paraffin tissue sections). Other applications not tested.
Recommended Dilution:The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used. Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：500ul
Source Antigen：Mouse ascites
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Reacts with chicken immunoglobulin. Some binding to human Fc receptors may occur. No crossreactivity with human cell surface or plasma components has been observed.
