DARPP-32 (dopamine and cyclic AMP regulated phosphoprotein, relative MR 32kD) is a cytosolic protein highly enriched in mediumsized spiny neurons of the neostriatum (1). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at threonine 34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (cdk5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype (3). DARPP-32 is a bifunctional signal transduction molecule that controls serine/threonine kinase and serine/threonine phosphatase activity depending on the phosphorylation state of the protein (2).
The Recombinant DARPP-32 His fusion protein preparation shows two bands of 38kD and 26kD, the latter representing a form lacking ~10–12kD from the carboxy terminus. Both bands are excellent substrates for phosphorylation.
Application:Suitable for use in Phosphatase assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant human DARPP-32, expressed in bacteria with a His-Tag linker.
Grade：Purified
Purity：~90% by SDS-PAGE.
Form：Supplied as a liquid in Tris-buffered saline, 40% glycerol.
