Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. Kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. With the BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin, enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration, i.e., the higher the OD the higher the BDNF concentration in the sample.
Sensitivity:7.8pg/ml
Range of Detection:7.8-500pg/ml
Crossreactivity:No significant crossreactivity with NGF, NT4/5 or NT3
Intra-assay Variation:± 3.7% (125pg/ml)
Inter-assay Variation:± 8.5% (125pg/ml)
Kit Components:There are enough reagents included in this kit for two 96-well immuno-assay plates
Storage and Stability:Store at 4°C. Do not freeze. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. *B2700-08D: BDNF Standard, Store at 4°C for up to 30 days (liquid).
