85-1381-52　Adenosine A1 Receptor, Rat, Positive Control 100ug　A0869-54

特徴

• Control peptide per A0869-52K (affinity purified antibody) and A0869-52D (antiserum).
• The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b and A3. The high affinity receptor A1 inhibits adenyl cyclase. Low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various, ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors. They are predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular. The C-termini is predicted to be cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1; q.31.3-32.2); A2a (Chromosome 22); A2b (Chromosome 17; p.11.2-12); A3 (Chromos.1).
• Applications:Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
• Recommended Dilution:ELISA: Use 50-100ng control peptide/ well
• Antibody Blocking: 5-10ug control peptide per 1ug A0869-52K (affinit purified antibody) or 1ul of A0869-52D (antiserum)Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.