His tagged mouse LIM Kinase 1, with an amino acid 1–14 deletion. MW 72kD
Specific Activity: ~130U/mg
Unit Definition: 1 Unit is equal to 1nmol phosphate incorporated into 10ug Cofilin 2 per minute at 30°C with a final ATP concentration of 100uM.
Quality Control Testing and Research Applications Kinase Assay:~50-400ng phosphorylates 10ug Cofilin 2 in vitro in the assay described.
MS Tryptic Fingerprint:Confirmed identity as LIM Kinase 1 with 28% amino acid coverage of the translated sequence listed
Storage and Shelf Life: Stable for 6 months at 20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated thawing and freezing.
仕様
Size：5ug
Source Antigen：Recombinant protein expressed in Sf21 cells
Grade：Highly Purified
Purity：≥ 90% by SDS-PAGE and Coomassie blue staining.
Form：Supplied as a liquid in 50mM Tris HCl pH 7.5, 150mM sodium chloride, 0.1 mM EGTA, 0.1% b-mercaptoethanol, 0.03% Brij-35, 1 mM benzamidine, 0.2mM PMSF, 50% glycerol.
