85-1380-88　Transferrin Receptor, soluble, Human, BioAssay™ ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR) 1Kit　T8199-38

特徴

• Human sTfR BioAssay™ ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum, plasma and tissue culture medium.
• Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.
• Principle:Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody’sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
• Specificity:Human soluble transferrin receptor
• Sample Volume:100ul/well
• Range:0.05-2ug sTfR /1ml of sample
• Sensitivity:2ng/ml
• Assay Time:The total assay time is less than 3 hours (incubation at the temperature of 30°C is necessary).
• Kit Components:T8199-38A: Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab T8199-38B: Mab (HRP), 1x13mlT8199-38C: Standard, 2ug/ml, 1x100ul T8199-38D: Standard, 1ug/ml, 1x100ul T8199-38E: Standard, 0.5ug/ml, 1x.100ul T8199-38F: Standard, 0.2ug/ml, 1x100ul T8199-38G: Standard, 0.1ug/ml, 1x100ul T8199-38H: Standard, 0.05ug/ml, 1x100ulT8199-38J: Quality Control, High, 50X, (5.5-8.3ug /ml), 1x50ulT8199-38K: Quality Control, Low, 50X, (1.3-2.0ug/ml), 1x50ulT8199-38L: Dilution Buffer, 2x13mlT8199-38M: Wash Solution, 10X, 1x100mlT8199-38N: Substrate Solution (TMB), 1x13mlT8199-38P: Stop Solution (0.2M H2SO4), 1x13ml
• Storage and Stability:Store kit at 4°C. Unopened kit components are stable for 6 months after receipt. Opened reagents are stable for 3 months when stored at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.