Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family. It is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108aa as a prepeptide. Its hydrophobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92aa polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan, et al., have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice. It is down regulated by fasting and antidiabetic drugs. Way, et al., on the other hand, have found that resistin expression is severly suppressed in obesity. It is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes. It also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.
Storage and Stability:Store all components at 4ºC. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
