85-1380-37　VspI 1000U　V4990

特徴

• 5'-A T^T A A T-3' 3'-T A A T^T A-5'
• Enzyme Properties:Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of DNA in 16 hours at 37°C.
• Thermal Inactivation: Enzyme is inactivated by incubation at 65°C for 20min.
• Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
• Compatible Ends: Csp6I, MaeI, NdeI, Tru1I
• Number of Recognition Sites in DNA:Lambda: 17PhiX174: 2M13mp18/19: 7pBR322: 1pUC18/19: 3pUC57: 3pTZ19R/U: 3pBluescriptIIKS(-/+): 3pBluescriptIISK(-/+): 3pACYC177: 2pACYC184: 1
• Supplied With: Provided to simply buffer selection
• R1625: Restriction Enzyme Buffer A, 10X
• R1625-03: Restriction Enzyme Buffer D, 10X.
• Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.