Form:Supplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
R1625 (double digests): Supplied as a liquid in 33mM Tris-acetate, (pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
Incubation Temperature:Incubate at 65°C. Incubate under paraffin oil in a capped vial. Incubation at 37°C results in 20% activity.
Inactivation Procedure:
To prepare prove for electrophoresis:-Stop the reaction by adding 2ul of 0.5M EDTA, pH 8.0, to achieve 20mM final concentration. Mix thoroughly, add an electrophoresis loading dye & apply to gel.
To prepare DNA suitable for further enzymatic reactions:-Extract with phenol/chloroform, precipitate with ethanol or isopropanol, wash the pellet with 75% cold ethanol and air dry;-Dissolve DNA in nuclease free H2O or TE buffer, or a buffer suitable for further applications
