85-1380-28　TaaI (Tsp4CI) 1000U　T0290

特徴

• 5'-A C N^G T-3' 3'-T G^N C A-5'
• Unit Definition:One unit is defined as the amount of Taal required to digest 1ug of lambda DNA in 1 hour at 65°C in 50ul of reaction buffer.
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X:Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• Diluent Buffer:10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol
• Storage Buffer:10mM Tris-HCl, pH 7.5, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol
• Methylation Effects:Dam: never overlaps - no effectDcm: never overlaps - no effectCpG: may overlap - cleavage impairedEcoKl: may overlap - effect not determinedEcoBl: may overlap - effect not determined
• Number of Recognition Sites in DNA:Lambda: 187, PhiX174: 15 pBR322: 14, pUC57: 8, pUC18/19: 8, pTZ19R/U: 7, M13mp18/19: 31
• Stability during Prolonged Incubation:A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 65°C.
• Compatible Ends:Hpy188l
• Incubation Temperature:65ºC
• Thermal Inactivation:Enzyme is inactivated by incubation at 80°C for 20 minutes.
• Overdigestion Assay:No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with TaaI.
• Ligation/Recleavage Assay:The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a facrtor of 100.
• Labeled Oligonucleotide (LO) Assay:No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide was observed after incubation with 10units of Taal for 4 hours.
• Storage and Stability:Store at -20°C. Aliquots are stable for 6 months after receipt.