85-1380-23　SmiI (SwaI) 200U　S1014-59

特徴

• 5'-A T T T^A A A T-3' 3'-T A A A^T T T A-5'
• Source:Streptococcus milleri S
• Concentration:10units/ul
• Unit Definition:One unit is defined as the amount of SmiI required to digest 1ug of Ad2 DNA-SspI fragments in 1 hour at 30°C in 50ul of assay buffer.
• Storage Buffer:10mM Tris-HCl (pH 7.4, 25°C) 200mM NaCl, 1mM DTT, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Supplied With:R1625 Restriction Enzyme Buffer A: 10X: 10mM Tris-HCl (pH 7.4, 25°C) 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. For longer periods, the Storage Buffer should be used.
• R1625-03 Restriction Enzyme Buffer D: 10X: 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.