85-1380-21　SdaI (Sse8387I) 1500U　S0549

特徴

• 5'-C C T G C A^G G-3' 3'-G G^A C G T C C-5'
• E. coli that carries the cloned sdaIR gene from Streptomyces diastaticus Ng 7-324
• Incubation Temperature:37°C
• Unit Definition:One unit is defined as the amount of Sdal required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of recommended reaction buffer.
• Storage Buffer:Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Diluent Buffer:10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate, pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA
• R1625-70: Restriction Enzyme Buffer for ScaI, SdaI, 10X: Dilute to 1X for use. 1X buffer composition is 37mM Tris-acetate, pH 7.0, 15mM magensium acetate, 150mM potassium acetate, 0.1mg/ml BSA
• Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.