85-1380-05　PdiI (NaeI) 1000U　P3121

特徴

• 5'-G C C^G G C-3' 3'-C G G^C C G-5'
• Concentration: 10u/ul
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X:Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• Diluent Buffer: 10mM Tris-HCl, pH, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 500mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 0.15% Triton X-100, 50% glycerol.
• Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with P3121.
• Ligation and Recleavage (L/R) Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test abililty to trace nuclease and phophatase activities with senstivity that is higher than L/R by a fctor of 100.
• Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide occurred during incubation with 10 units of Pdil for 4 hours.
• Blue/White Cloning Assay: The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phophatase activities with sensitivity that equals to that of B/W test.
• Methylation Effects: No effect on Dam, Dcm, EcoKl and EcoBl. Overlaps and blocks CpG.