85-1379-99　PaeI (SphI) 500U　P1800

特徴

• Sequence:5'-G C A T G^C-3' 3'-C^G T A C G-5'
• Source: Pseudomonas aeruginosa
• Concentration: 10u/ul
• Unit Definition:One unit is defined as the amount of PaeI required to digest 1ug of lambda DNA fragments in 1 hr at 37°C in 50ul assay buffer.
• Diluent Buffer:Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Storage Buffer:10mM potassium phosphate (pH 7.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA, 0.15% Triton X-100 and 50% glycerol.
• Supplied with:R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37ºC.
• R1625-01: Restriction Enzyme Buffer B, 10X: Supplied as a liquid 10mM Tris-HCl, pH 7.5 at 37°C, 10mM magnesium chloride, 0.1mg/ml BSA. Incubate at 37°C
• Double Digests:R1625 is provided to simplify buffer selection for double digests. 98% of PaeI, works well in a 1x or 2x.