85-1379-95　NheI 1000U　N2425

特徴

• 5'-G^C T A G C-3' 3'-C G A T C^G-5'
• Source:Neisseria mucosa heidelbergensis
• Unit Definition:One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-HindIII fragments in 1 hour at 37°C in 50ul of assay buffer.
• Concentration:10 units/ul
• Supplied With:R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA (pH 7.9 at 37°C) when diluted to 1X.
• Diluent Buffer:10mM Tris-HCl, (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Storage Buffer:Supplied as a liquid in 10mM Tris-HCl, pH 8.0 at 25ºC, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Incubation Temp: 37°C
• Compatible Ends:BcuI, Eco130I, XbaI, XmaJI
• Number of Recognition Sites in DNA Molecules:Lambda: 1PhiX174: 0M13mp18/19:0pBR322: 1pUC18/19: 0pUC57: 0pTZ19R/U: 0
• *Note:Supercoiled plasmids may require up to 10-fold more Nhel for complete digestion than linear DNAs (e.g. 10units are required to cleave 1ug of pBR322 DNA).
• Thermal Inactivation:NheI is inactivated by incubation at 65°C for 20min.
• Stability During Prolonged Incubation:≥ 0.2units of NheI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37ºC.
• Enzyme Properties:Methylation Effects on Digestion:Dam, Dcm, EcoKl, EcoBl: never overlaps - no effectCpG: May overlap - cleavage impaired
• Digestion of Agarose-embedded DNA:A minimum of 5 units of Nhel is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16hrs