85-1379-91　Mva1269I (BsmI) 200U　M9581

特徴

• 5'-G A A T G C N↓-3' 3'-C T T A C↑G N-5'
• Unit Definition:One unit is defined as the amount of Mva1269I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
• Incubation Temperature:37°C
• Dilution Buffer:10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid containing 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA
• R1625-04: Restriction Enzyme Buffer E, 10X: Supplied as a liquid in 10mM Tris-HCl, pH 8.5, 10mM magnesium chloride, 100mM potassium chloride, 0.1mg/ml BSA.
• Thermal Inactivation:Mva1269I is inactivated by incubation at 65°C for 20min.
• Enzyme Properties:Methylation Effects:Dam, Dcm, EcoKI: Never overlaps - no effectCpG: May overlap - no effectEcoBI: May overlap - effect not determined
• Stability during Prolonged Incubation: A minimum of 0.1units of Mva1269I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
• Digestion of Agarose-embedded DNA: A minimum of 5 units of Mva1269I is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
• Number of Recognition Sites in DNA:Lambda: 46PhiX174: 4pBR322, pUC57, M13mp18/19: 1pUC18/19, pTZ19R/U: 0
• Thermal Inactivation:Mva1269I is inactivated by incubation at 65°C for 20min.
• Enzyme Properties:Methylation Effects:Dam, Dcm, EcoKI: Never overlaps - no effectCpG: May overlap - no effectEcoBI: May overlap - effect not determined
• Stability during Prolonged Incubation: A minimum of 0.1units of Mva1269I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
• Digestion of Agarose-embedded DNA: A minimum of 5 units of Mva1269I is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
• Number of Recognition Sites in DNA:Lambda: 46PhiX174: 4pBR322, pUC57, M13mp18/19: 1pUC18/19, pTZ19R/U: 0