85-1379-89　［受注停止］MunI (MfeI) 1500U　M9219

特徴

• 5'-C^A A T T G-3' 3'-G T T A A^C-5'
• Concentration:~10u/ul
• Source:E. coli that carries the cloned munIR gene from Mycoplasma unidentified.
• Storage Buffer:Enzyme is supplied in 10mM Tris-HCl pH7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
• Diluent Buffer:10mM Tris-HCl pH7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Supplied with:R1625 - Restriction Enzyme Buffer A, 10X:Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• R1625-02 - Restriction Enzyme Buffer C, 10X:Dilute to 1X for 100% MunI digestion. 1X buffer compostion is 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM sodium chloride and 0.1mg/ml BSA. Incubate at 37°C
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap