85-1379-85　Mph1103I (AvaIII) 1000U　M4680

特徴

• Sequence:5'-A T G C A^T-3' 3'-T^A C G T A-5'
• Source:Moraxella phenylpyruvica RFL1103
• Concentration: 10u/ul
• Unit Definition:One unit is defined as the amount of Mph1103I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X:Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• R1625-04: Restriction Enzyme Buffer, 10X:Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM potassium chloride,, 0.1mg/ml BSA
• Storage Buffer:Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Enzyme Properties:Stability during Prolonged Incubation: A minimum of 0.3 units of Mph1103I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
• Methylation Effects:Dam/Dcm/CpG/EcoKl: never overlaps- no effectEcoBl: may overlap-effect not determined
• Thermal Inactivation:Mph1103I is inactivated by incubation at 65°C for 20min.
• Compatible Ends: Alw21I, PstI, SdaI, SduI
• Number of Recognition Sites in DNA:Lambda: 14PhiX174: 0M13mp18/19: 0pBR322: 0pUC18/19: 0pUC57: 1pTZ19R/U: 0
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.