85-1379-83　MluI 1000U　M4140

特徴

• 5'-A^C G C G T-3' 3'-T G C G C^A-5'
• Source:Microcccus luteus
• Concentration: ~10u/ul
• Unit Definition:One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
• Incubation Temperature: 37°C
• Diluent Buffer: 10mM Tris-HCl, pH 7.4 at 25°C, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol, or Storage Buffer.
• Storage Buffer: 10mM Tris-HCl, pH 7.5 at 25°C, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Supplied With:R1625: Restriction Enzyme Buffer A, 10XR1625-04: Restriction Enzyme Buffer E, 10X
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.