Unit Definition:One unit is defined as the amount of KpnI required to digest 1ug of lambda DNA-BamHI fragmants in 1 hour at 37°C in 50ul of assay buffer
Supplied With:R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-65: Restriction Enzyme Buffer for KpnI, 10X: Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 10mM MgCl2, 0.02% Triton X-100 and 0.1mg/ml BSA. Incubate at 37°C.