Concentration: 1u/ulSource: E.coli that carries the cloned gsuIR gene from Gluconobacter suboxydans H-15T store at 20°C
Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2 and 0.1mg/ml BSA. Incubate at 30°C**
Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer term storage, the Storage Buffer should be used.
Storage Buffer: 10mM potassium phosphate (pH 7.5 at 25°C), 1mM EDTA, 7mM 2-mercapthoethanol, 0.2mg/ml BSA and 50% glycerol.