85-1379-48　Eco52I (XmaIII) 2500U　E0374-25

特徴

• 5'-C^G G C C G-3'3'-G C C G G^C-5'
• Sequence:5'-C^G G C C G-3'3'-G C C G G^C-5'
• Source:Escherichia coli RFL52
• Concentration:10u/ul
• Unit Definition:One unit is defined as the amount of Eco52I required to digest 1ug of lambda DNA-Eco81I fragments in 1 hour at 37°C in 50ul of recommended reaction buffer.
• Form:Supplied as a liquid in 10mM Tris-HCl, pH 8.2, 500mM sodium chloride, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X:Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• R1625-60: Restriction Enzyme Buffer, 10X:Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl, pH 8.5, 3mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA.
• Thermal Inactivation:Enzyme is inactivated by incubation at 65°C for 20 minutes.
• Diluent Buffer:10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Enzyme Properties:Methylation Effects:Dam, Never overlaps - no effectDcm: Never overlaps - no effectCpG: Completely overlaps - blockedEcoKI: Never overlaps - no effectEcoBI: Never overlaps - no effect
• Stability during Prolonged Incubation:A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
• Digestion of Agarose-embedded DNA:A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
• Number of Recognition Sites in DNA:Lambda: 2PhiX174: 0pBR322: 1pUC57: 0pUC18/19: 0pTZ19R/U: 0M13mp18/19: 0
• Compatible Ends:Bsp120I, CfrI, NotI
• Overdigestion Assay:No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion of Eco52I (10u/ug lambda DNA x 16 hours).
• Ligation/Recleavage (L/R) Assay:The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.
• Labeled Oligonucleotide (LO) Assay:No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed during incubation with 10 units of Eco52I for 4 hours.
• Blue/White Cloning Assay:The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test.
• Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.