85-1379-42　Eco31I 1000u　E0373-55

特徴

• 5'-G G T C T C(N)1↓-3'3'-C C A G A G(N)5↑-5'
• E. coli that carries the cloned eco31IR gene from E. coli RFL31
• Concentration:10u/ul
• Unit Definition:One unit is defined as the amount of Eco31l required to digest 1ug of lambda DNA dcm--HindIII fragments in 1 hour at 37°C in 50ul of reaction buffer. Assayed using lambda DNA dcm-, as one of two Eco31I recognition sites is difficult to cleave.
• Storage Buffer:10mM Tris-HCl, pH 7.5, 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X:Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA
• R1625-02: Restriction Enzyme Buffer C, 10X, Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5 10mM magnesium chloride, 50mM sodium chloride, 0.1mg/ml BSA.
• Incubation Temperature:37ºC
• Thermal Inactivation:Eco31l is inactivated by incubation at 65°C for 20 minutes
• Overdigestion Assay:No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion with Eco31I (5u/ug lambda DNA dcm- x 16 hours).
• Ligation/Recutting Assay:The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.
• Labeled Oligonucleotide (LO) Assay:No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Eco31I for 4 hours.
• Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.