85-1379-39　Eam1104I (Ksp632I) 1500U　E0200

特徴

• 5'-C T C T T C(N)1^-3' 3'-G A G A A G(N)4^-5'
• Concentration: 10u/ul.
• Source:Enterobacter amnigenus RFL1104
• Unit Definition:One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
• Incubation Temperature:37°C
• Dilution Buffer:10mM Tris-HCl, pH 7.4 at 25°C, 100mM potassium chloride, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Storage Buffer:Supplied as a liquid in10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Supplied With:R1625: Restriction Enzyme Buffer A, 10X. Provided to simply buffer selection.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.