5'-G^T A C-3' 3'-C A T^G-5'Unlike RsaI, Csp6I produces DNA fragments with a 2-base 5'-extension.
Source:Corynebacterium species RFL6
Concentration:10u/ul
Unit Definition:One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
Storage Buffer:Supplied as a liquid in 10mM Tris-HCl, pH 7.4 at 25°C, 100mM potassium chloride, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Supplied With:R1625 Restriction Enzyme Buffer A, 10X: Supplied as a liquid in33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37°C.
R1625-01 Restriction Enzyme Buffer B, 10X: Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 10mM magnesium chloride, 0.1mg/ml BSA. Incubate at 37°CEnzyme Properties:Stability during Prolonged Incubation:A minimum of 0.1u of Csp6I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C
Thermal Inactivation: Csp6I is inactivated by incubation at 65°C for 20min
Compatible Ends: FspBI, NdeI, Tru1I, VspI Number of Recognition Sites in DNA:Lambda: 113PhiX174: 11pBR322, pUC57, pUC18/19: 3pTZ19R/U: 2M13mp18/19: 19Methylation Effects:Dam, Dcm, EcoKI, EcoBI: Never overlaps-no effectCpG: May overlap-no effectQuality Control:Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with Csp6I
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Csp6I more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 05uM. More than 95% of these can be recut. Labeled Oligonucleotide Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10u of Csp6I for 4 hours.
仕様
Size：1500U
Source Antigen：Corynebacterium species RFL6
Grade：Molecular Biology Grade
Form：Supplied as a liquid in 10mM Tris-HCl, pH 7.4 at 25°C, 100mM potassium chloride, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
