85-1379-18　Bsp119I (AsuII) 2500U　B2940

特徴

• Sequence:5'-T T^C G A A-3' 3'-A A G C^T T-5'
• Concentration: 10u/ul
• Source: Bacillus species RFL119
• Storage Buffer:Supplied as a liquid in10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Diluent Buffer:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X:Suplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• Enzyme PropertiesCompatible Ends:Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp11406I, Ssil, TaqI, XmiI
• Methylation Effects:Dam, Dcm, EcoBI: Never overlaps - No effect
• CpG: Completely overlaps - Blocked
• EcoKI: May overlap - Effect not determined.
• Stability during Prolonged Incubation:A minimum of 0.1units of Bsp119I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
• Thermal Inactivation:Bsp119I is inactivated by incubation at 80°C for 20min.
• Digestion of Agarose-embedded DNA:A minimum of 5units of Bsp119I is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
• Number of Recognition Sites in DNA:Lambda: 7 PhiX174, M13mp18/19, pBR322, pUC18/19, pUC57, pTZ19R/U, pTZ57R,pBluescriptIIKS(+/-), pBluescriptIISK(+/-): 0pACYC177: 1pACYC184: 2