85-1379-11　Bsh1236I (FnuDII) 500u　B2922

特徴

• 5'-C G↓C G-3' 3'-G C↑G C-5'
• Source:Bacillus sphaericus RFL1236
• Concentration:10u/ul
• Incubation Temperature:37°C
• Storage Buffer:Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Diluent Buffer:10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA
• R1625-04: Restriction Enzyme Buffer E, 10X: Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl, pH 8.5 at 37°C, 10mM MgCl2, 100mM KCl, 0.1mg/ml BSA
• Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.