85-1379-03　BseDI (SecI) 300U　B2905

特徴

• 5'-C^C N N G G-3' 3'-G G N N C^C-5'
• Source: Bacillus stearothermophilus RFL1434
• Concentration: 10u/ul
• Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 55°C in 50ul of assay buffer.
• Dilution Buffer: 10mM Tris-HCl, pH 7.4 at RT, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.5 at RT, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Directions For Use: Incubate at 55°C. (Incubation at 37°C results in 10% activity.)
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X. Suitable for use in Double Digest assays.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.