85-1379-01　BpiI (BbvII) 1000U　B2640

特徴

• 5'-G A A G A C (N)2∨-3' 3'-C T T C T G (N)6^-5'
• Supplied With:R1625: Restriction Enzyme Buffer A, 10X, Dilute to 1X. 1X buffer compostion is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• R1625-02: Restriction Enzyme Buffer C, 10X, Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2, 50mM sodium chloride, 0.1mg/ml BSA.
• Unit Definition:One unit is defined as the amount of BpiI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of reaction buffer.
• Diluent Buffer:10mM Tris-HCl, pH 7.4 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Storage Buffer:10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Enzyme Properties:Overdigestion Assay:No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BpiI.
• Ligation/Recutting Assay:The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.
• Labeled Oligonucleotide (LO) Assay:No detectable degradation of single-stranded or double-stranded labeled oligonucleotide occurred duringincubation with 10 units of Bpil for 4 hours.
• Blue/White Cloning Assay:The B/W assay was relpaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test.
• Methylation Effects:Dam, Dcm, EcoKl: never overlaps - no effectCpG: may overlap - no effectEcoBl: may overlap - effect not determined
• Stability during Prolonged Incubation:A minimum of 0.3units of BpiI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
• Thermal Inactivation:BpiI is inactivated by incubation at 65°C for 20 minutes.
• Digestion of Agarose-embedded DNA:A minimum of 5units of BpiI is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
• Number of Recognition Sites in DNA:Lambda: 24 PhiX174: 3 pBR322: 3 pUC57: 0 pUC18/19: 0 pTZ19R/U: 0 M13mp18/19: 0
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.