85-1378-94 [取扱停止]BfuI (BciVI) 100U B1086-05
特徴
- Sequence:5'-G T A T C C (N)6^-3' 3'-C A T A G G (N)5^-5'
- Source: Bacillus firmus Auk
- Concentration: 5u/ul
- Unit Definition:One unit is defined as the amount of BfuI required to digest 1ug of lambda DNA dcm in 1 hour at 37°C in 50ul of reaction buffer.
- Supplied With:R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA (pH 7.9 at 37°C).
- R1625-20: Restriction Enzyme Buffer for Bful, 10X: Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25°C), 300mMNaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
- Storage and Dilution Buffer:10mM Tris-HCl (pH 7.4 at 25ºC), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
- Thermal Inactivation:Bful is inactivated by incubation at 80°C for 20min.
仕様
- Size:100U
- Grade:Molecular Biology Grade
- EU Commodity Code:38220090
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