85-1378-86　Alw26I (BsmAI) 1000U　A1372-10

特徴

• 5'-G T C T C (N)1^-3' 3'-C A G A G (N)5^-5'
• Source:Acinetobacter lwoffi RFL26
• Concentration:10u/ul
• Unit Definition:One unit is defined as the amount of Alw26I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
• Incubation Temperature:37°C
• Diluent Buffer:10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Storage Buffer:Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
• Restriction Enzyme Buffer A, 10X:R1625: Supplied as a liquid in 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C. Supplied to simplify buffer selection for double digests.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.