Concentration: 10u/ul Unit Definition:One unit is defined as the amount of AdeI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
Incubation Temperature: 37°C
Diluent Buffer:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer:Supplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Supplied with:R1625-02: Restriction Enzyme Buffer C, 10X:Dilute to 1X. 1X buffer compostion is 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM sodium chloride and 0.1mg/ml BSA. Incubate at 37°C
R1625: Restriction Enzyme Buffer A, 10X:Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
Enzyme Properties:Star Activity:An excess of AdeI (20u/ug DNA x 1 hour) may result in star activity.
Methylation Effects:Dam, Dcm: Never overlaps - No effect
CpG: May overlap - cleavage impaired
EcoKl, EcoBl: May overlap - Effect not determined.
Stability during Prolonged Incubation:A minimum of 0.5units of AdeI is required for complete digestion of 1ug of DNA in 16 hours at 37°C.
Digestion of Agarose-embedded DNA:A minimum of 10units of AdeI is required for complete digestion of 1ug of agarose- embedded lambda DNA in 16 hours.
Thermal Inactivation:AdeI is not inactivated by incubation at 80°C for 20min (see Protocol for Inactivation Procedure
Number of Recognition Sites in DNA:Lambda: 10 PhiX174, pTZ19R/U, M13mp18/19: 1pBR322, pUC18/19, pUC57: 0
