85-1378-79　AatII 1500U　A0002

特徴

• Sequence:5'-G A C G T^C-3' 3'-C^T G C A G-5'
• Supplied With:Restriction Enzyme Buffer A (R1625): (for 100% AatII digestion) 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C.
• Diluent Buffer:10mM Tris-HCl (pH 7.4 at 25°C), 100mM potassium chloride, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Storage Buffer:10mM Tris-HCl (pH 7.4 at 25°C), 100mM potassium chloride, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
• Overdigestion Assay:No detectable change in the specific fragmentation pattern is observed after a 160-fold overdigestion with AatII (10u/ug lambda DNA x 16 hours).
• Ligation/Recutting Assay:After 50-fold overdigestion (3u/ug DNA x 17 hours) with AatII more than 95% of the digested DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these sites can be recut.
• Labeled Oligonucleotide (LO) Assay:No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.