85-1378-73　XhoI 2000u　X1030

特徴

• 5'-C↓T C G A G-3' 3'-G A G C T↑C-5'
• Form:Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• R1625 (double digests): Supplied as a liquid in 33mM Tris-acetate, (pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Incubate at 37°C
• R1625-04 (for 100% digestion): Supplied as a liquid in 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37°C.
• Unit Definition:One unit is defined as the amount of XhoI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
• Protocol for Digestion:Add:Nuclease free water: 16ulR1625-04: 2ulDNA (0.5-1ug/ml): 1ulX1030: 0.5-2ulMix gently and spin down for a few seconds. Incubate at 37ºC for 1-16 hours.
• Protocol for Digestion Directly after Amplification:Add:PCR Reaction Mixture: 10ul (~0.1-0.5ug of DNA)Nuclease free water: 18ulR1625-04: 2ulX1030: 1-2ulMix gently and spin down for a few seconds
• Incubate at 37ºC for 1-16 hours.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.