Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion with HinfI (10u/ug lambda DNA x 16 hours) .
Ligation/Recutting Assay:After 50-fold overdigestion (3u/ug DNA x 17 hours) with HinfI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.3uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay:No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide occured during incubation with 10 units of HinfI for 4 hours.
Methylation Effects:Dam: never overlaps-no effectDcm: never overlaps-no effectCpG: may overlap-cleavage impairedEcoKI: never overlaps-no effectEcoBI: may overlap-blocked
Stability during Prolonged Incubation:A minimum of 0.1 units of HinfI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
