85-1378-25　BsuRI (HaeIII) 3000u　B3100

特徴

• 5'-G G^C C-3' 3'-C C^G G-5'
• Source:Bacillus subtilis R
• Diluent Buffer:10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Thermal Inactivation:BsuRI is inactivated by incubation at 80°C for 20 minutes.
• Storage Buffer:10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• R1625-04: Dilute to 1X for 100% BsuRI digestion. 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37°C
• Incubation Temperature: 37°C
• Unit Definition:One unit is defined as the amount of BsuRI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
• Methylation Effects:Dam, EcoKl, EcoBl: never overlaps - no effect
• Dcm, CpG: may overlap - no effect.
• Protocol for Digestion:Add:Nuclease free water: 16ulR1625-04: 2ulDNA (0.5-1ug/ml): 1ulB3100: 0.5-2ulMix gently and spin down for a few seconds. Incubate at 37ºC for 1-16 hours.
• Protocol for Digestion Directly after Amplification:Add:PCR Reaction Mixture: 10ul (~0.1-0.5ug of DNA)Nuclease free water: 18ulR1625-04: 2ulB3100: 1-2ulMix gently and spin down for a few seconds
• Incubate at 37ºC for 1-16 hours.
• Storage and Stability:Aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.