Unit Definition:One unit is defined as the amount of PsuI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of recommended reaction buffer.
Form:Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.
Supplied with:R1625: Restriction Enzyme Buffer A, 10X:Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
Supplied with R1625-01 Restriction Enzyme Buffer B, 10X:Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2, 0.1mg/ml BSA.
Incubation Temperature:37ºC
Thermal Inactivation:PsuI is inactivated by incubation at 80°C for 20 minutes.
Overdigestion Assay:No detectable change in the specific fragmentation pattern is observed after a 80-fold overdigestion with PsuI (5u/ug lambda DNA x 16 hours).
Ligation and Recleavage (L/R) Assay:The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.
Labeled Oligonucleotide (LO) Assay:No detectable degradation of single-stranded or double-stranded labeled oligonucleotides occurred during incubation with 10 units of PsuI for 4 hours.
Enzyme Properties:Methylation Effects:Dam, Completely overlaps - no effectDcm: May overlap - no effectCpG: May overlap - no effectEcoKI: Never overlaps - no effectEcoBI: May overlap - effect not determined
Stability during Prolonged Incubation: A minimum of 0.5units of PsuI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Compatible Ends:BamHI, BclI, Bsp143I, MboI, BglII
Number of Recognition Sites in DNA:Lambda: 21PhiX174: 0pBR322: 8pUC57: 7pUC18/19: 7pTZ19R/U: 7M13mp18/19: 3
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
