85-1378-22　Bsp143I (MboI, Sau3AI) 300U　B2950

特徴

• Description:5'-^G A T C-3' 3'-C T A G^-5'
• Source:Bacillus species RFL143
• Concentration: 10u/ul
• Unit Definition:One unit is defined as the amount of Bsp143I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
• Diluent Buffer:For short term storage (3-4 weeks)- dilute with 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
• Storage Buffer:For long term storage: 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA, 50% glycerol.
• Supplied With:R1625-35 Restriction Enzyme Buffer for Bsp143I, 10X:Suplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 0.02% Triton X-100, 0.1mg/ml BSA.
• R1625: Restriction Enzyme Buffer A, 10X:Suplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
• Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer